Development of one-tube multiplex polymerase chain reaction (PCR) for detecting <i>Mycobacterium bovis</i>
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چکیده
منابع مشابه
Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis
A multiplex PCR (m-PCR) with primers targeting the 16S rRNA, Rv3873 and a 12.7-kb fragment in the genomes of a Mycobacterium tuberculosis complex was designed for the differential diagnosis of M. tuberculosis, M. bovis, M. bovis BCG and non-tuberculosis Mycobacterium (NTM). The specificity of this assay was 100%, and the detection limit was 15 pg of genomic DNA. Of the 206 blinded clinical samp...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2016
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.15-0216